Founded in 2009,[1] it is the second entirely pediatric hospital in the country, the other being CURE Children's Hospital of Uganda (CCHU), located in Mbale, in Eastern Uganda, which was founded in 2000.

[2] HICH is located in the city of Mbarara, in Mbarara District, approximately 3 kilometres (2 mi) west of Mbarara Regional Referral Hospital, the largest hospital in the Western Region of Uganda, and the nearest regional referral hospital.

[3] This location is approximately 272 kilometres (169 mi), by road southwest of Mulago National Referral Hospital in Kampala, Uganda's capital and largest city.

[4] The hospital is a collaborative effort between many stakeholders, including the following:[1] Holy Innocents Children's Hospital is a member of the Uganda Catholic Medical Bureau.

Patient user fees accounted for 45.3 percent of the total hospital annual income.