Holybrook is a civil parish, forming a contiguous part of Reading in West Berkshire and is a mixture of urban, suburban land with watercourses and flood meadows in Berkshire, England.

The parish takes its name from the Holy Brook, a watercourse which forms its southern boundary and which is a corollary of the River Kennet.

Its main settlements are part of Calcot those commonly known as Beansheaf Farm and Fords Farm, Holybrook and occasionally considered part of Calcot or Southcote which overlaps with this area.

[3] The civil parish dates from 2000 when it was created from the part of Theale east of the M4 motorway.

The parish council and the unitary authority are responsible for different aspects of local government.