Hong Kong St. John Ambulance (Chinese: 香港聖約翰救護機構; Jyutping: hoeng1 gong2 sing3 joek3 hong3 gau3 wu6 gei3 kau3; Cantonese Yale: heūng góng sing yeuk hong kau wuh geī kau) is a charitable organisation with a long history stretching back over a century and has been serving the community since 1884.

Adhered to its motto, "For the Service of Mankind", the organisation is dedicated to providing first aid and ambulance services in emergency, dental care for the handicapped, and courses on first aid and home nursing for the general public.

Dr. Wai-Cheung Chau (周懷璋,1893–1965), was present in the battle at Magazine Gap as a medical officer of St John Ambulance.

St. John Ambulance Brigade, the service arm of St. John, is responsible for the organisation, training and equipment of men, women and young persons to undertake, on a voluntary basis, either as individuals or as organised groups, First Aid, Home Nursing and allied activities in public places, or elsewhere as occasions may require, for welfare to those in need.

It is hoped, too, that the Cadet Command will lay the foundation for membership of the Ambulance and Nursing Divisions of the Brigade.

It was then reorganized and renamed as the Bagpipe Team and incorporated into the Support Region in 2020, until it was dissolved by Administrative Order on April 1 of the same year.

The St. John Ambulance Headquarters is at 2 MacDonnell Road, Mid-Levels, Hong Kong Island,[6] which has been reconstructed to accommodate a hotel to guarantee a financial income.

The Hong Kong Island Command headquarters has since moved to 2 Tai Hang Road, Causeway Bay in a historical building built in 1935, overlooking the Central Library and Victoria Park.

The New Territories' headquarters is located at 28 Tin Ping Road in Sheung Shui, mainly as an ambulance depot.