St John Ambulance Northern Territory (St John NT) is a non-profit, charitable organisation providing first aid services and training, urgent care, patient transport, ambulance and other medical services in the Northern Territory.

[3] A first aid course was held in Darwin in September/October 1915 with successful students being awarded a certificate from the St John Ambulance Association.

In 1952 Mr Charles Bannerman wrote to Sydney HQ about restarting St John Ambulance in Darwin.

Around the same time, officers from the Department of Civil Aviation (DCA) were working on forming a branch of the St John Ambulance Brigade.

A meeting was held in Darwin on 28 April 1952 with DCA Fire Chief Norman Bradbury elected as president.

As early as 1971 the Department of Health was considering that St John should operate the full Darwin ambulance service, which would need paid staff.

St John took over the ambulance service from the Department of Health in Alice Springs and in Tennant Creek on 1 July 1979.

Of the most common of ambulances is the Mercedes-Benz Sprinter which serves as the organisation's primary means of emergency patient transport.

Two Ford Ranger Operational Support Vehicles are in service equipped to manage mass-casualty incidents in remote areas.

The service is also introducing Toyota HiAce vans fitted with suspension lifts, bull bars, and a 4x4 conversion for Nhulunbuy and other remote areas.