Hoplitis tuberculata exhibits a disjunct distribution range, with its habitat spanning the subalpine zones of the Alps and extending to boreal regions in Europe and Asia.

[2] The species primarily nests in insect borings in dead wood and utilizes various materials for constructing its brood cells.

[2] Notable features of its nest include three-layered partitions between brood cells, consisting of masticated leaf walls enclosing an interlayer densely packed with pebbles, earth crumbs, and small particles.

[2] Brood cells discovered in the Autumn contain larvae encased in a semi-transparent, brownish-white cocoon, indicating that Hoplitis tuberculata overwinters in a prepupal stage.

It has been discovered to harvest pollen from at least ten different plant families, including Fabaceae, Ericaceae, Rosaceae, Ranunculaceae, Asteraceae, Asparagaceae, and Violaceae.