Hoshina Masatoshi (保科 正俊) (1509–1593) was a Japanese samurai of the Sengoku period, who served the Takeda clan.

Masatoshi served under Shingen from the latter's earliest campaigns and fought in many of battles.

He initially opposed Takeda Shingen's invasion of Shinano; however, he later submitted to Shingen and became a Takeda general, in command of 120 cavalry.

Together with Sanada Yukitaka and Kōsaka Masanobu, he was one of the three "Danjo" (Danjō stands for a formal title, Danjōchū; 弾正忠) in the Takeda clan, distinguished from the others as "Yari Danjō" (槍弾正), due to his skilled use of the spear.

Masatoshi was succeeded by his son Hoshina Masanao.