According to several media reports, by the end of 2018 Hospitallers had evacuated or treated up to 2750 Ukrainian soldiers, during the on-going Russo–Ukrainian war.

[4][3] In a later interview Yana described how fierce battles for Ukrainian villages, Karlivka and Pisky, made her think of the necessity of a volunteer medical unit.

[6][8][4] When Dmytro Yarosh formed the Ukrainian Volunteer Army (UDA) in 2015, "Hospitaliers" became its structural division.

[6] The Hospitallers are operating in the war zone to provide assistance to the Ukrainian military, both volunteer units and the official Armed Forces.

[4] In 2015, one of the battalion's paramedics, Yevhen Titarenko, and Natalia Khazan filmed a movie "War for Peace" based on 120 hours of documentary footage shot at the front line.

[14] The Hospitallers continue their work during the Russian invasion of Ukraine in 2022,[12] accept new volunteers,[12] and are fundraising for vehicle repairs, fuel and medical supplies.