Hubie and Bertie represent some of animator Chuck Jones' earliest work that was intended to be funny rather than cute.

[3] The plot of the cartoon would serve as the template for most future Hubie/Bertie outings: a character with some mental illness or degree of naïveté (here, a cat who doesn't know what a mouse looks like) is psychologically tormented by the pair.

Jones would repeat the theme of mind games several more times in his Hubie and Bertie shorts, as in their third cartoon, Roughly Squeaking on November 23, 1946.

By the short's end, the cat thinks he is a lion, the dog believes he is a pelican, and a bystanding bird (driven mad from watching the two) has pulled his feathers out and imagines himself a Thanksgiving turkey.

In the next cartoon, Mouse Wreckers, and for the remainder of the series, Blanc and Freberg would handle the voices of Hubie and Bertie, respectively.

In the cartoon, the duo moves into a new home, only to discover that it is protected by champion mouser Claude Cat (the character's debut), voiced by Mel Blanc.

After these seven cartoons, Jones retired Hubie and Bertie, but continued to use the characters (or mice resembling them) in cameo roles in other shorts whenever he needed a generic mouse for a gag, such as the unnamed mouse in Chow Hound, who resembles Bertie, or the "killer" mice in Scaredy Cat.