The eldest son of Hugh Chamberlen the elder, he was educated at Trinity College, Cambridge, and took the degree of Master of Arts (MA) in 1683 per literas regias.
[1] Chamberlen's only published work was a Latin epithalamium, written on the marriage of Princess Anne in 1683.
[1] A monument to Chamberlen, put up by the 2nd Duke of Buckingham and Normanby, is in the north choir aisle of Westminster Abbey.
His life size effigy reclines in doctoral robes on the lid of a sarcophagus surrounded by emblematic sculptures, while a long Latin epitaph by Atterbury praises his family, his life, his descendants, and his patron.
[1] Chamberlen's library was sold in 1734 after the death of his widow, and a copy of the catalogue went to the British Museum.