Sir Hugh Hamersley (6 July 1565 – 19 October 1636) was a 17th-century merchant who was Lord Mayor of London in 1627.

Hamersley's family estate was at Pyrton Manor near Watlington, Oxfordshire, but he rose to prominence in London as a merchant.

In 1627 he became Lord Mayor of London and served another term as Master of the Haberdashers Company.

[1] After Hamersley's death a memorial was erected by his widow in the church of St Andrew Undershaft, London.

The Ashbourne portrait is also believed to represent Hamersley, and not, as long thought, William Shakespeare.