A. Milne's books, the term "Hundred Acre Wood" is actually used for a specific part of the larger Forest, centred on Owl's house (see the map in the book, as well as numerous references in the text to the characters going "into" or "out of" the Hundred Acre Wood as they go between Owl's house and other Forest locations).

In the games, the main character, Sora, gathers pages of a Winnie-the-Pooh storybook after it is destroyed, affecting the inhabitants.

The Hundred Acre Wood is unique in being a totally optional world to visit and entirely lacking in combat, rather being made up of several minigames which reward the player with experience and items.

In the 2023 horror film Winnie-the-Pooh: Blood and Honey, the inhabitants of the Hundred Acre Wood suffer from a famine after Christopher Robin leaves for college.

Pooh, Piglet, Owl and Rabbit consume Eeyore in order to survive, although the trauma causes them to revert back to their animalistic instincts and develop a hatred towards Christopher and the rest of humanity.