It started serialization on Flex Comix's free web comic FlexComix Blood on July 12, 2006.

Hyakko takes place in the high school division of Kamizono Academy (上園学園, Kamizono Gakuen), a fictional gigantic private institution located in Kyūshū, Japan, in which the motto is "to bring up talents of students in all departments and fields".

Its storyline focuses on the comedic school life of the four main heroines, Torako Kageyama, Ayumi Nonomura, Tatsuki Iizuka, and Suzume Saotome.

As the plot progresses, Torako and her friends gradually begin to learn and interact with their classmates in Class 1–6, in which each student has a unique and extraordinary personality.

Right Stuf Inc. licensed the series in North America and released it on DVD under their Lucky Penny label on April 2, 2013.