Mic60/Mitofilin (encoded by the IMMT gene) is a core subunit of the MICOS-complex, directly located next to cristae junctions (CJ).

Human Mic60 exists in two isoforms of different size, anchored to the IMM via its N-terminus, while most of the protein is located to the inner mitochondrial space (IMS).

Mic60 is evolutionary one of the oldest MICOS subunits as homologous were found in anaerobic prokaryotes.

This subcomplex, especially Mic60, is crucial for the physical contact between the IMM and the outer mitochondrial membrane (OMM) via its interaction with SAM50.

Through its interaction with TOM and TIM, Mic60 additionally influences the import of precursor proteins.