Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium.
[1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar, resulting in the formation of a visible precipitate when they interact.
[1][2] Immunodiffusion techniques are widely used in immunology for various purposes, including:[1][2] In this method, antibodies are uniformly distributed in an agar gel, and the antigen sample is placed in wells cut into the gel.
As the antigen diffuses radially, it forms a precipitation ring with the antibody.
[3][2] This method involves both antigen and antibody diffusing through the gel from separate wells, forming precipitation lines where they meet and react.