I-664 begins at a full Y interchange with I-64 and I-264 that serves as the terminus of all three Interstates in the Bower's Hill section of the city of Chesapeake.

The rail spur leaves the median and heads northeast toward Portsmouth just south of its interchange with SR 164 (Western Freeway) and US 17 (Bridge Road).

[1][2] North of SR 135, northbound I-664 has a vehicle inspection station and crossovers before the highway enters the MMMBT.

I-664 heads north-northeast along a causeway for three miles (4.8 km) to a point west of the Newport News Middle Ground Light, where the pair of bridges curve to the north-northwest onto an artificial island where the highway descends into a pair of tunnels under the estuary's main shipping channel.

[1][2] I-664 has a southbound vehicle inspection station adjacent to its first interchange in Newport News with Terminal Avenue.

The Interstate parallels the southern end of CSX's Peninsula Subdivision as it passes through interchanges with several streets to the east of downtown Newport News.

The Interstate enters the city of Hampton and has diamond interchanges with Aberdeen Road and Powhatan Parkway before reaching its northern terminus at I-64.

I-664 meets its parent highway at a directional Y interchange above Newmarket Creek just south of Hampton Coliseum.