It began in downtown Cedar Rapids at 1st Avenue near Interstate 380 and ended at County Road E36 near Palo, Iowa.

Due to the proximity of the Cedar River and that the southernmost one-half mile (800 m) of the highway was on one-way streets, the northbound lanes began on the western bank of the river while the southbound lanes ended on the eastern bank.

Once it exited the Cedar Rapids city limits, the highway was known as Covington Road.

Iowa 94 ended at a T-intersection with CR E36 on the western edge of Palo.

The eastern terminus was at what today is known as U.S. Highway 30 near Mount Vernon, Iowa.