Isola d'Asti is a comune (municipality) in the Province of Asti in the Italian region Piedmont, located about 45 kilometres (28 mi) southeast of Turin and about 8 kilometres (5 mi) south of Asti.

Isola d'Asti borders the following municipalities: Antignano, Asti, Costigliole d'Asti, Mongardino, Montegrosso d'Asti, Revigliasco d'Asti, and Vigliano d'Asti.

The municipal seat is in the frazione of Isola Piano.

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