Born in Bergamo in 1530[1] to Isotta Ludovico and Lucas Brembati,[2] Brembati was fluent in Greek, Latin, French, Spanish, and Italian.

[3] Initially married to Count Lecio Secco d'Aragona di Castro, she remarried to Gian Gerolamo Grumelli in 1561.

Brembati and Grumelli gained significant local influence as a couple and were painted twice by Giovanni Battista Moroni.

[4] Brembati gained widespread acclaim for her poetry.

After her death in 1586, a book of poems titled Rime funerali di diversi illustri ingegni composte in volgare et latina favella in morte della molto illustre signora Isotta Brembati-Grumelli was published to commemorate her.