Nine members of the delegation participated in the games for the second time: Lonah Chemtai Salpeter, Gili Cohen, Shlomi Haimy, Sagi Muki, Andrea Murez, Shira Rishony, Or Sasson, Marhu Teferi, and Maor Tiyouri.

Seven athletes from the delegation had previously participated in the Youth Olympic Games: Linoy Ashram, Gal Cohen Groumi, Artem Dolgopyat, Anastasia Gorbenko, Denis Loktev, Avishag Semberg, and Yakov Toumarkin.

[7] One Israeli archer qualified directly for the men's individual recurve at the Games, by reaching the quarterfinals and obtaining one of the seven available spots at the 2021 Final Qualification Tournament in Paris, France.

[8] Israel fielded a squad of two artistic swimmers to compete in the women's duet event, by finishing sixth at the 2021 FINA Olympic Qualification Tournament in Barcelona, Spain.

Misha Zilberman and Ksenia Polikarpova were selected to compete at the Games each in the men's and women's singles based on the BWF World Race to Tokyo Rankings.

Three-time Olympian Alexander Shatilov booked a spot in the men's individual all-around and apparatus events, by finishing seventh out of the 12 gymnasts eligible for qualification at the 2019 World Championships in Stuttgart, Germany, while Artem Dolgopyat secured one of the three places available for individual-based gymnasts, neither part of the team nor qualified through the all-around, in the floor exercise at the same tournament.

[25] Lihie Raz received a spare berth from the women's apparatus event, as one of the female gymnasts from the individual all-around, not yet qualified, at the 2019 World Championships.

Linoy Ashram and Nicol Zelikman secured two quotas for the Israeli delegation by finishing third and eleventh, respectively, at the 2019 World Championships all-around individual event.

Israel qualified a squad of 12 judoka (six men and six women) for each of the following weight classes at the Games by the IJF World Ranking List of 28 June 2021.

Gil Cohen and Noa Lasry secured a quota for the Israeli delegation by finishing eleventh in the women's 470 event at the 2018 Sailing World Championships in Aarhus, Denmark.

Yoav Cohen finished in fifth place at the 2021 RS:X World Championships and tied with Shahar Tzuberi in Israel's selection ranking.

Shahar Tibi and Noya Bar Am were chosen to represent Israel in the 470 event, while Kakon was selected in the women's Laser Radial.

Israeli shooter Sergey Richter achieved quota places for the delegation in the men's 10 metre air rifle, by winning the gold medal at the 2019 European Games.

Avishag Semberg secured a spot in the women's flyweight category (49 kg) with a top-two finish at the 2021 European Qualification Tournament in Sofia, Bulgaria.

Brothers Ran and Shachar Sagiv were selected among the top 26 triathletes competing in the men's event based on the ITU World Rankings.