Commissioned into service in the Italian Regia Marina (Royal Navy) in 1915, she served in World War I, playing an active role in the Adriatic campaign and seeing action in the Battle of the Strait of Otranto in 1917.

[2] Arranged in a radial pattern, the three destroyers first followed the route between the position of the attack and the Austro-Hungarian Navy base at Cattaro, then zigzagged in a northerly direction, and then headed south.

[2] On 29 December 1915 Antonio Mosto was among a number of Allied warships that put to sea to intercept an Austro-Hungarian force composed of the scout cruiser Helgoland and the destroyers Balaton, Csepel, Lika, Tátra, and Triglav which had bombarded the harbor at Durrës (known to the Italians as Durazzo) on the coast of the Principality of Albania, sinking the Greek steamer Mikael and two sailing ships while losing Lika, which struck a mine.

[3] On 13 June 1916 Antonio Mosto and the destroyers Audace, Pilade Bronzetti, and Rosolino Pilo provided escort and support to the motor torpedo boats MAS 5 and MAS 7, which, after the coastal torpedo boats 35 PN and 37 PN towed them to a starting position, penetrated the harbor at Austro-Hungarian-occupied Shëngjin (known to the Italians as San Giovanni di Medua) in Albania.

[4] On the night of 14–15 May 1917, the Battle of the Strait of Otranto, the largest naval action of the Adriatic Campaign of World War I, began when the Austro-Hungarian Navy staged a two-pronged attack against the Otranto Barrage aimed both at destroying naval drifters — armed fishing boats that patrolled the anti-submarine barrier the barrage formed — and, as a diversionary action, at destroying an Italian convoy bound from Greece to Albania.

At 04:50 on 15 May, following news of these attacks, Antonio Mosto, the Italian destroyer Giovanni Acerbi, and the British light cruiser HMS Bristol got underway to intervene in the clash, heading northeast to intercept the Austro-Hungarian ships.

Bristol, the British light cruiser HMS Dartmouth, Antonio Mosto, and Giovanni Acerbi placed themselves between Aquila and the Austro-Hungarian ships and opened fire on them at 09:30 at a range of 8,500 metres (9,300 yd).

All the major warships suffered damage during the battle, but Antonio Mosto′s formation had to discontinue the action and withdraw at 12:05 when it neared Cattaro, from which the Austro-Hungarian armored cruiser Sankt Georg and destroyers Tátra and Warasdiner had sortied to intervene in the engagement.

[4] An Austro-Hungarian Navy force consisting of the scout cruiser Helgoland and the destroyers Balaton, Csepel, Lika, Orjen, Tátra, and Triglav left Cattaro on 18 October 1917 to attack Italian convoys.

[8] Antonio Mosto, under the command of Tenente di vascello (Ship-of-the-Line Lieutenant) Aldo Crugnola, was moored at Tripoli on 9 July 1941 during a British air raid.

[7] At 13:00 on 28 March  1942, while under the command of (Deputy Corvette Captain) Gerolamo Delfino, she got underway from Patras, Greece along with the auxiliary cruiser Città di Napoli, the destroyer Sebenico, and the torpedo boats Angelo Bassini, Castelfidardo, and San Martino to escort a convoy composed of the troopships Aventino, Galilea, Francesco Crispi, Italia, Piemonte, and Viminale headed to Brindisi and then on to Bari, Italy, loaded with 8,300 troops.

[9] Francesco Crispi, Galilea, Piemonte, and Viminale were transporting elements of the 3rd Alpine Division "Julia" returning to Italy from Greece, while Aventino and Italia were carrying men from the garrisons of the Italian Dodecanese headed home on leave.

[9] As ordered before departure from Patras, the rest of the convoy continued its voyage, leaving only Antonio Mosto behind to counterattack Proteus with depth charges and assist Galilea.

Antonio Mosto avoided capture by the Germans, moving first to Palermo, a port in the hands of the Allies, and then on 20 September 1943 to Malta along with many other Italian ships.