Like its predecessor and the original television show, the film is a compilation of stunts, pranks and skits, starring the regular Jackass cast of Johnny Knoxville, Bam Margera, Dave England, Ryan Dunn, Chris Pontius, Wee Man, Steve-O, Preston Lacy, and Ehren McGhehey.

The film opens by introducing the primary cast members while bulls aggressively chase them in a suburban neighborhood, wreaking havoc in the process.

The most notable stunts include: Johnny Knoxville riding a big red rocket which gets launched into the air; Bam Margera being trapped and stuck in a trailer with a king cobra; Dave England riding a giant firehose that launches him into a patch of mud; Ryan Dunn being launched into a garage door while riding a shopping cart; Chris Pontius' penis dressed as a mouse while a snake attempts to bite it; Wee Man getting zapped by an electric stool upon believing that cards are being thrown at him by champion card thrower Jim Karol; Steve-O getting his cheek pierced by a fish hook and getting thrown into the shark-infested Gulf of Mexico; Preston Lacy disguising himself as Bam's father Phil, in order to spoon with Bam's mother April in bed; and Ehren McGhehey attempting a loop while riding a pocketbike.

The movie concludes with Johnny Knoxville putting his hand in a bear trap, before transitioning into a performance by the main cast in a Busby Berkeley-style movie musical production number set to the La Cage aux Folles song "The Best of Times", where the guys sing and dance while performing more stunts.

Crew members who appear in this movie: Stunts including Viva La Bam regular Don Vito were also filmed and shown in original trailers.

However, due to the scandal surrounding his arrest just prior to the film's release and the nature of the charges against him, all the scenes involving Don Vito were cut late in development.

Notable exceptions to the supporting cast are Raab Himself, who was battling alcoholism and drug addiction at the time, and Rake Yohn, who did however appear in Jackass 2.5.

Even after completing enough footage for Number Two, Knoxville was so energized by being back together with his friends and shooting again, that he encouraged everyone to stay together and film more stunts (most of which ultimately released with 2.5), willing to even put himself at further risk of injury simply because he didn't want to part with the group so soon.

Deleted scenes that were also filmed but not included in either the final film or Jackass 2.5 include: "The Lamborghini Tooth Pull" with Don Vito (which would later be performed in Jackass 3D by Ehren McGhehey); "The Human Newton's Cradle", where Preston Lacy, Bam Margera, Phil Margera, Don Vito, and Brandon DiCamillo were the "spheres" for a life size Newton's cradle; "Meatball Sling Shot", where Bam slingshots a meatball in Vito's face (which was also performed by Phil Margera and included in Jackass 2.5); "SWAT Team Wake Up", where a SWAT Team wakes Johnny Knoxville up by blowing the door to his motel room; "Wee Baby", where Wee Man is dressed as a baby and put in a box in an attempt to get shipped out through the mail (which was later redone in Jackass Presents: Bad Grandpa); "Unicycle Hellride", where Steve-O rides a unicycle over hot coals; "Balloon Flight", where Bam has balloons tied to him which causes him to float in the sky, he then uses a pellet gun to shoot the balloons with to slowly descend into a lake; and "Ignited Farts", where Bam, Steve-O, Ryan Dunn, Chris Pontius, and Dave England would get in a bathtub and try to ignite their farts underwater, ending with Dave defecating by accident (this stunt would later be performed by Steve-O in Jackass Forever).

[14] The New York Times awarded Jackass Number Two a Critic's Pick, writing, "Debased, infantile and reckless in the extreme, this compendium of body bravado and malfunction makes for some of the most fearless, liberated and cathartic comedy in modern movies.

Both DVDs feature commentary by the cast (with the exception of Bam Margera), director and producer Jeff Tremaine, and co-producer and cinematographer Dimitry Elyashkevich.

The DVD also included 16 deleted scenes removed from the theatrical release, more than 20 additional scenes, 9 TV spots, 8 promotional spots including the theatrical trailer, gag reel, the uncensored version of Karazy by Chris Pontius and a promotional commercial for the 2006 VMAs.

[19][20] The most notable bits include: Johnny Knoxville getting a prostate exam and trying to donate sperm at a clinic in Russia; Bam Margera flying a kite out of his anus with anal beads; toy airplanes attacking Preston Lacy while he is painted as King Kong; Dave England and Ehren McGhehey boxing with after getting violently spun on office chairs; Wee Man bullfighting against a calf; a snapping turtle biting Chris Pontius on the nose; Steve-O drinking beer off Shridhar Chillal's nails; Ryan Dunn and Bam getting golf balls teed off of them, and members of the Jackass team going through a Cajun obstacle course with alligators, pigs, paintball shots, and spittoon drinking.