Together with the [New Haven Warriors], the Axemen were founded in 2006 as an expansion team of the American National Rugby League (AMNRL).

They are one of the most successful teams in America both in on-field and off-field operations,[2] making it to the finals in four of their five years of play.

After becoming the first AMNRL club to come from the southern state the Axemen had an impressive debut season finishing in the top six and qualifying for the playoffs; however they would eventually be knocked out by the New Jersey Sharks.

The Jacksonville Axemen hosted the teams of the Australia Day Challenge[4] on January 26, 2008 at the University of North Florida, Hodges Stadium.

They hold the current record for most fans and supporters at home games with average crowds of 2500 and have merchandise sales that ship globally during the season.

In January 2011 the Axemen were one of seven AMNRL teams to announce they were departing to form the new USA Rugby League.

The club managed to secure the inaugural minor premiership, however disappointingly were eliminated in the first week of the play-offs by the New Haven Warriors.

They did, however, go on to win the National Championship in only their second year in the new league (2012) On August 25, 2018, the Jacksonville Axemen defeated the Brooklyn Kings 58–16 to win the 2018 National Championship Top Row: Sean Rutgerson (Coach), Jed Pearce, Lachlan Bristow, Jon Alexander, Steven Hill, Khalial Harris, James Campbell, Hunter Lawson, David Thomas, Kris Townsel, Glenn Spigelmyer, David Washington, Desta Bailey, Dr. B. Hudson Berrey (Team Physician) Middle Row: Mary Slover (Head Athletic Trainer), Kyle Grinold, Montana Northcroft, Taylor “Thor” Youngling, Jon Purnell (C), Tyler “Sparky” McClain, Will Fletcher, Kevin Wathen, Alex Eberle Bottom Row: Haley Frazer (Athletic Trainer), Jessika Wojciechowski (AT Intern), Kayla Jones (Athletic Trainer), Leslie Quirk (AT Intern)

a cross code game between Rugby League and American Football, by playing the Jacksonville Knights and defeated them 38-27[8] It may have been the first time that both an American Football team and a Rugby League team played a cross code game.