In the following year he published several works against the eminent Italian jurist, Pietro Tomasi of Ravenna, who was then lecturing in the German universities.
While van Hoogstraten took no active part in the earlier stages of the controversy, his sympathies were with Reuchlin's opponents as evidenced by his close relationship with Pfefferkorn.
Influenced no doubt, to some extent by the unfavourable attitude of the universities towards the Jewish books, van Hoogstraten on 15 September 1513, in his capacity as inquisitor, summoned Reuchlin to appear within six days before the ecclesiastical court of Mainz to answer to the charges of favouring the Jews and their anti-Christian literature.
Meanwhile, van Hoogstraten had Reuchlin's Augenspiegel, a previously published retort to Pfefferkorn's Handspiegel, publicly burned at Cologne.
On 29 March 1514, the Bishop of Speyer announced that the Augenspiegel contained nothing injurious to the Catholic Faith, pronounced judgment in favour of Reuchlin, and condemned van Hoogstraten to pay the expenses consequent upon the process.