He was the son of Anne de Bueil and Pierre d'Amboise, lord of Chaumont, as their seventh of eighteen children.
Entering the Benedictine order he was elected abbot of Jumièges in January 1474, replacing his brother Louis on his appointment as bishop of Albi.
[1] Located beside the Gallo-Roman ruins, the hôtel de Cluny hosted several royal visits and would be inhabited by cardinal Mazarin in 1634.
[citation needed] The bishopric of Clermont had been left vacant at the end of 1504, and Louis XII appointed Jacques d'Amboise to it – he held the post from 1505 until his death.
[1] He completed the diocese's cathedral and replaced its tiles with a lead roof, as well as richly adding to its library and creating a tapestry in the choir of which four pieces are now in St Petersburg.