[2] Jacqui Day was born in Mountain Creek, Queensland,[3] and grew up in the Sunshine Coast.

[4] Throughout her junior career Jacqui Day represented her home state, Queensland, in national tournaments.

In 2013, she culminated her junior career at the Under–21 Australian Championships in Darwin, and was adjudged Player of the Tournament.

[5] Following her performance at the 2013 national championships, Day was named in a 25–player squad for the Junior World Cup, however did not make the final 18–player team.

[9][8] Jacqui Day made her senior international debut for the Hockeyroos in 2016, during a test match against China in Perth.