He had been in contact with Team Saxo Bank, and the deal was finally signed the day before he won Danmark Rundt.

Despite having signed the contract, Fuglsang's manager wanted him to wait as his market value could inflate if he won Danmark Rundt which he did the following day.

Fuglsang rode his first World Tour season in 2009, riding for Team Saxo Bank which was led by Bjarne Riis.

In his last race for Team Saxo Bank, Fuglsang finished 4th in Giro di Lombardia after beating Vincenzo Nibali in a sprint.

Fuglsang rode Amstel Gold Race where an attack on Cauberg saw Philippe Gilbert take the victory.

He was slated to be his team's leader in the 2012 Giro d'Italia, but had to withdraw due to knee problems and was replaced with Fränk Schleck.

Having phoned the general manager Johan Bruyneel, it was confirmed that Fuglsang was not riding any more World Tour races in 2012.

Throughout the spring of the 2014 season, Fuglsang showed he had consistent form at the major World Tour stage races.

However, on the final sector of cobblestones, Nibali was once again having problems, and could not close the gap to Lars Boom who rode away in the distance and won the stage.

[18] His main goal of the season was to help Vincenzo Nibali defend his 2014 Tour de France title.

Rodríguez won the stage with 1:12 down to Jakob Fuglsang who arrived at the soaking wet finish line in 2nd place.

After making a comeback in the final week of the race, Nibali ended up winning the Giro d'Italia with help from Fuglsang and his Astana team.

At the 2016 Olympic Games in Rio de Janeiro Fuglsang was in the chaser group descending the final climb, when two of the main-favorites Vincenzo Nibali and Richie Porte crashed.

In June 2017 Fuglsang rode the Critérium du Dauphiné alongside Italian team-mate Fabio Aru as the leader.

Aru reached the top of the climb first with Chris Froome, Richie Porte and Jakob Fuglsang 15 seconds behind.

Froome and Team Sky did everything to win and attacked early on the stage which cracked race leader Porte.

Eventually Fuglsang reached the downhill with the other general classification contenders as they hunted down Warren Barguil for the stage win.

Fuglsang attacked inside the final 500 meters but was chased down by Rigoberto Urán and lost his chance for the stage win.

Fuglsang arrived at Paris-Nice with hopes of riding for the general classification, however he was involved in a crash on the first stage, and lost time as he did the previous year.

His next attempt to improve form after the opening stage races of the year was the first altitude training camp for the Tour de France.

Fuglsang and his Astana team-mates got off to a very bad start to the race, only finishing 20th in the team time trial on stage 1.

At the Canadian World Tour classic Grand Prix Cycliste de Montréal, Fuglsang was in a late breakaway with Tim Wellens (Lotto–Soudal) and James Knox (Quick-Step Floors), but was brought back by the bunch with 6.2 kilometers to go.

At Amstel Gold Race, Fuglsang rode away with Alaphilippe, and they looked to fight out the win, however they slowed down drastically in the final kilometre which allowed the chasing group to bridge the gap to the leaders.

He attacked in the final stages and dropped everyone, however he nearly crashed with 3 kilometres to go, but a miraculous save from Fuglsang secured him his first win in a monument classics.

Thibaut Pinot tried an attack inside the final two kilometres, however Fuglsang quickly closed it down, and won his second Critérium du Dauphiné.

After the racing resumed in August, Fuglsang started with a 5th-place finish in the Italian neo-classic Strade Bianche,[28] and a week later, he managed to come 2nd in the Tour de Pologne.

[30] The victory in Lombardy was Fuglsang's second monument win,[31] and it also marked the first time a Danish cyclist won the race.

Alaphilippe escaped on the last climb and soloed to victory, while Fuglsang lost the sprint for silver and bronze medals in a chase group.

[42] He finished third overall for the second year in succession at the Tour de Suisse, also spending a day in the leader's jersey.

[45] Fuglsang rode only four races in the first half of 2023, finishing only two – the Tour de Hongrie,[46] and the Mercan'Tour Classic,[47] both held in May.