Sir James Boleyn (died 1561) was a courtier in the reign of Henry VIII of England and chancellor of the household of his niece, Anne Boleyn, the second wife of Henry VIII.
He was thus the grand-uncle of Elizabeth I. James was the son of Sir William Boleyn and his wife, Margaret Butler.
This position did not involve regular attendance at court and was "largely honorific".
James married Elizabeth Wood, who was one of the principal witnesses against their niece, Anne Boleyn, when she was arrested for adultery, incest and conspiring to kill the king.
James is described as someone who shared Anne Boleyn's reformist beliefs.