The James Madison Dukes women's lacrosse team is an NCAA Division I college lacrosse team representing James Madison University as part of the American Athletic Conference.

They play their home games at Sentara Park in Harrisonburg, Virginia.

In 2018, the Dukes won the National Championship, beating Boston College 16-15.

With JMU having moved most of its other sports to the Sun Belt Conference, which does not sponsor women's lacrosse, in July 2022, the Dukes joined the American Athletic Conference as an affiliate member at that time.

Division regular season champion Division regular season and conference tournament champion