Jana Klusáčková (née Myšková) (born 29 October 1977) is a Czech former swimmer who specialized in sprint freestyle events.

She also participated in the women's 4 × 100 m freestyle relay, along with fellow swimmers Sandra Kazíková, Petra Klosová, and Ilona Hlaváčková.

Swimming the lead-off leg, Klusackova recorded a split of 56.02 seconds, and the Czech team continued to finish heat one in seventh place, and thirteenth overall, for a total time of 3:46.83.

[8][9] At the 2008 Summer Olympics in Beijing, Klusackova qualified for the second time in the women's 100 m freestyle by breaking a new Czech record and clearing a FINA B-cut of 55.41 from the national championships in Prague.

[10] She challenged seven other swimmers in the fifth heat, including top favorites Cate Campbell of Australia, Francesca Halsall of Great Britain, and Aliaksandra Herasimenia of Belarus.