[1] Chambers was born in Columbia, South Carolina, but grew up in Orlando, Florida, where she started writing with scripts for local public radio stations.
After studying acting for a season at the Pasadena Playhouse in 1956, she moved to New York City and then on to Poland Spring, Maine, where she worked for WMTW.
A Late Snow, produced at Playwrights Horizons in 1974 was one of the earliest plays to portray lesbian characters in a positive light.
In 1980, Chambers started to work with The Glines, writing Last Summer at Bluefish Cove for their First Gay American Arts Festival, about the impact upon a woman and her lesbian friends after she is diagnosed with cancer.
She continued to write, producing My Blue Heaven for the Second Gay American Arts Festival at the Glines, and The Quintessential Image for the Women's Theatre Conference in Minneapolis.