[1] For surface running, the boats were powered by two 500-brake-horsepower (373 kW) diesel engines, each driving one propeller shaft.
[4] Upon commissioning, Ro-114 was attached to the Kure Naval District and was assigned to Submarine Squadron 11 for workups.
[4] On 20 December 1942, her commanding officer received orders to attend the Kure Submarine School to take a class on the new Type 92 electric torpedo.
[4] On 7 February 1944, Ro-114 was reassigned to the headquarters of the Grand Escort Command to conduct antisubmarine operations in the Ryukyu Islands.
[4] On 13 June 1944 the Combined Fleet activated Operation A-Go for the defense of the Mariana Islands, and that day the commander-in-chief of the 6th Fleet, Vice Admiral Takeo Takagi, ordered all 18 submarines available to him to deploy east of the Marianas.
[4] On 17 June 1944, the United States Navy destroyers USS Melvin (DD-680) and USS Wadleigh (DD-689) detected a submerged Japanese submarine on sonar in the Philippine Sea 80 nautical miles (150 km; 92 mi) west of Tinian.
[4] On 12 July 1944, the Imperial Japanese Navy declared Ro-114 to be presumed lost in the Philippines area with all 55 men on board.