Jarmila Novotná (September 23, 1907 in Prague – February 9, 1994 in New York City) was a Czech lyric coloratura soprano and actress.
A student of Emmy Destinn, Novotná made her operatic debut at the National Theatre in Prague, on June 28, 1925, as Mařenka in Smetana's The Bartered Bride.
In 1929 she joined the Kroll Opera in Berlin, where she sang Violetta as well as the title roles of Puccini's Manon Lescaut and Madama Butterfly.
In January 1933, she created the female lead in Jaromír Weinberger's new operetta Frühlingsstürme, opposite Richard Tauber at the Theater im Admiralspalast, Berlin.
She appeared as Pamina in the 1937 Salzburg Festival production of Mozart's The Magic Flute, conducted by Arturo Toscanini.