Jean Holmes-Mitchell (born 7 November 1940) is a Panamanian sprinter.
She competed in the women's 100 metres at the 1960 Summer Olympics.
She finished second in the 1959 Pan American Games 4 × 100 metres Relay (with Carlota Gooden, Marcela Daniel, and Silvia Hunte).
At the 1963 Pan American Games Holmes finished fourth in the 100 metres event.
This biographical article relating to Panamanian athletics is a stub.