[citation needed] After graduating from Boston University, Kline interned at Roger Corman's Concorde/New Horizons and worked in feature development for Michael Shamberg and Harold Ramis's Ocean Pictures.

He served as a television executive in Daytime Programming at NBC Entertainment for one year then spent five years at Columbia Pictures Television, eventually serving as the senior vice president of drama before transitioning into writing/producing at the suggestion of his first writing partner, Frank Lupo, co-creator of The A-Team and Wiseguy.

[citation needed] From 2006 to 2008, he wrote a Paris Hilton pilot for MTV, and co-wrote the feature film The Rosenbergs Save Christmas (with Goldie Hawn attached to star) for Fox Searchlight.

Kline's Darby Pop Productions produced Jingle Bell Rocks, a documentary about Christmas music, distributed by Oscilloscope Laboratories.

[8] In 2018, Kline served as Executive Producer and Head Writer on a new series (10) of Woody Woodpecker animated shorts for YouTube.

The crew of Transformers Prime Beast Hunters has been nominated for a 2014 Daytime Emmy Award for Outstanding Special Class Animated Program for which Jeff Kline was named for his work as executive producer.

The series Transformers: Prime won a 2012 Daytime Emmy Award for Outstanding Special Class Animated Program for which Jeff Kline was named for his work as executive producer.

[citation needed] Kline's shows have also been nominated for Daytime Emmys in: 2007: My Friends Tigger & Pooh 2002: Jackie Chan Adventures; Harold and the Purple Crayon won for Outstanding Achievement in Main Title Design; MIB: The Animated Series won for Outstanding Sound Editing Special Class 2001: Jackie Chan Adventures, Roughnecks: Starship Troopers Chronicles 2000: MIB: The Animated Series; Roughnecks: Starship Troopers Chronicles won for Outstanding Sound Editing Special Class 1999: MIB: The Animated Series.