She is known for her roles as Jennifer Husser on the TNT series Claws (2017), Mackenzie Bradford-Lopez on the FX sitcom Saint George (2014), Lindsey Salazar on the FX drama Justified (2010), and most recently Esther Finch in Netflix's series Dead Boy Detectives (2024).

[2][3] Upon graduation, Lyon moved to New York where she worked in theatre and formed her own comedy sketch troupe POYKPAC in 2006.

[2][4] Her work with POYKPAC, led to her co-starring in the IFC web comedy television series Good Morning Internet!.

[8] Lyon married her long-term partner Taige Jensen at the Astoria World Manor in Queens in October 2019.

[9] Lyon has opened up about her experiences with disordered eating and receiving treatment from the Renfrew Center.