[2] Lowe was born in Raleigh, North Carolina, and along with his brother Enos, the family soon afterward moved to the Indiana Territory, settling in Monroe County.
After studying law and interning with Tilghman Howard in Indiana, Lowe decided not to become a lawyer, instead serving in the commissary of a Missouri volunteer regiment under Sterling Price.
On July 3, 1853, Lowe crossed the Missouri River with his brother Enos into Indian Territory, estimating the land across from Council Bluffs was going to become a great city.
Lowe was a founding member of the Omaha Claim Club in 1854, and assisted Alfred D. Jones in the first survey of the city.
The Jesse Lowe Conference Room, named in his honor, was located on the third floor of the Civic Center.