Jiřina Šejbalová (17 September 1905 – 23 August 1981) was a Czech actress of the stage, television, and film.

For many years she taught theatre classes at the Janáček Academy of Music and Performing Arts.

After studying at the Prague Conservatory, she began her career as an opera singer at the National Theatre in Brno in the mid-1920s.

During the 1927-1928 season she abandoned her opera career and began working as a dramatic actress at that house in avant-garde plays directed by Jiří Frejka.

Šejbalová made her first foray into Czech film as the title character in the 1929 movie Hanka a Jindra.