John Bothwell of Auldhamer, Lord Holyroodhouse (c.1550–1609) was a 16th-century Scottish judge and Senator of the College of Justice residing at Holyrood House prior to it becoming a royal palace.
His paternal uncle Richard Bothwell served as Provost of Edinburgh during the reign of Mary Queen of Scots.
His grandfather Francis Bothwell had also been a Senator of the College of Justice since its foundation in 1532 and a Lord of Session in the Edinburgh courts.
[1] He dressed as an Amazon in August 1594 to perform in the tournament at the baptism of Prince Henry at Stirling Castle.
[6] His correspondence with Anthony Bacon in England in 1596 mentions his relation, Mr Kello, the husband of the calligrapher Esther Inglis.