John Chamberlain (c. 1560 - 1617) of Prestbury, Gloucestershire was an English politician.
[1] He was the son of the diplomat Sir Thomas Chamberlain of Prestbury, Gloucestershire.
[2] He married Elizabeth, daughter of Sir John Thynne, but had no children.
[1] He has been identified as the man who sat for Clitheroe in 1593 and St Germans in 1597.
This article about a 16th-century member of the parliament of England is a stub.