Through a friend Eyre was able to enter Trevecca College, and with the Countess of Huntingdon's Connexion he ministered at Tregony, Cornwall, Lincoln, and Mulberry Gardens Chapel, London.

About the end of 1785 year Eyre was appointed minister of Homerton, often then called Ram's Chapel after its founder and he opened a school at Well Street, Hackney.

A scheme originated in 1796 by Eyre and others to sending out evangelical preachers into the counties south of London, and from this arose Hackney Theological College, opened in 1803.

William Jay noted how Eyre, a Calvinist in theology, was asked to preach at John Wesley's chapel in Moorfields, and gave no offence.

[1] After a long illness Eyre died on 28 or 29 March 1803, and was buried in a vault on the south side of the communion-table in Homerton Chapel, 5 April.