John Frelinghuysen (1727 – September 5, 1754) also known as Johannes Frelinghuysen was a minister in colonial New Jersey whose work in education laid the groundwork for the establishment Rutgers University (as Queen's College in 1766) and the New Brunswick Theological Seminary (in 1784).

John preached in the revivalistic style of Calvinism that his father was known for as part of the First Great Awakening.

John lived in the Old Dutch Parsonage in Somerville where he served the three local congregations until his death.

He took in students and a room in the house served as a Dutch Reformed religious seminary.

This center of education was a forerunner of the New Brunswick Theological Seminary and Queen's College, which later developed into Rutgers University.