He was licensed as a preacher by the presbytery of Dunkeld, and soon afterwards ordained by that of Dundee as minister of the parish of Tealing (1719), where his preaching soon drew a large congregation.
Early in his ministry he was brought to a halt while lecturing on the Shorter Catechism by the question "How doth Christ execute the office of a king?"
This led to an examination of the New Testament foundation of the Christian Church, and in 1725, in a letter to Francis Archibald, minister of Guthrie, Forfarshire, he repudiated the obligation of national covenants.
From the scriptural doctrine of the essentially spiritual nature of the kingdom of Christ, Glas in his public teaching drew the conclusions that: This argument is most fully exhibited in a treatise entitled The Testimony of the King of Martyrs (1729).
The seat of this congregation was shortly afterwards transferred to Dundee (whence Glas subsequently removed to Edinburgh), where he officiated for some time as an elder.
About thirty Churches of Christ were established in Great Britain through the efforts of Glas and others, including his son in law, Robert Sandeman.
The Testimony of the King of Martyrs concerning His Kingdom (1729) is a classic repudiation of erastianism and defence of the spiritual autonomy of the church under Jesus Christ.
His common sense appears in his rejection of John Hutchinson's attempt to prove that the Bible supplies a complete system of physical science, and his shrewdness in his Notes on Scripture Texts (1747).