John Gyles (died c. 1406) of Dover, Kent, was an English politician.
He had two sons, Peter, a clerk, and Thomas, an MP and king's esquire.
By 1366, he was a tavern owner and in 1368 he was fined 10s for stabbing a man with a dagger.
He attended the coronation of Richard II of England as a canopy bearer.
He was a Member (MP) of the Parliament of England for Dover in 1385, 1386, February 1388, September 1388, January 1390, 1391, 1393, 1395 and 1399.