John Trelawny (or Trelawney) (died 14 October 1568), of Pool in Menheniot, Cornwall, was an English landowner.
The son of another John Trelawny of Pool, who had been a co-heir of the Earl of Devon, he was the head of one of Cornwall's leading families.
He was also High Sheriff of Cornwall in 1561 and 1567, and a Deputy Lieutenant, and was paid the sum of £6 for not taking the order of knighthood.
His eldest son, John (died 1569), was an infant when he inherited his father's estate and lived only a few months afterwards, but the inheritance passed to a second son, Jonathan (later Sir Jonathan, 1568–1604), born after his father's death.
His widow was remarried to William Mohun.