Sir John Villers (1485/1486 – 8 December 1544), of Brooksby Hall, Leicestershire, was an English politician.
In 1520 he was with Henry VIII at the Field of Cloth of Gold and the meeting with Charles V at Gravelines and in 1522 served in the Picardy campaign.
He returned to France ten years later for Henry VIII's meeting with Francis I at Calais.
He was elected a Member (MP) of the Parliament of England for Leicestershire in 1539.
With no legitimate male heir, his bequeathed his lands at Brooksby and elsewhere to his brothers and an illegitimate son.