Sir John Wollaston (died April 1658) was an English merchant who was Lord Mayor of London in 1643.
Wollaston was a city of London merchant and a member of the Worshipful Company of Goldsmiths.
On 5 February 1639 he was elected an alderman of the City of London for Farringdon Without ward and was Prime Warden of the Goldsmiths Company from 1639 to 1640.
In 1642 he became alderman for Dowgate ward and colonel of the Yellow Regiment, London Trained Bands.
He founded, in 1658, by bequest, almshouses for six poor women, and endowed them with a rent-charge of £18 10s.