The main protagonist of the series' sixth story arc, Stone Ocean, Jolyne is falsely accused of murder by Dio's most loyal friend, Enrico Pucci, and sentenced to 15 years in prison.

Eventually, from a pendant given to her by her father, Jotaro Kujo, she acquires her Stand, Stone Free (ストーン・フリー, Sutōn Furī),[a] which gives her the ability to unravel her body into string.

[1][2] Araki's editor during Stone Ocean's publication, Hideto Azuma, suggested that Jolyne be changed to a man as they could lose the interest of readers.

[1] In creating Jolyne's theme song for the anime, Yugo Kanno explained that since she is the first female protagonist he composed for, he wanted to harmonize her femininity and powerfulness and found it challenging.

[18] Realizing the extent of how much her father loved her and his desire to keep her safe, she vows to stay in order to save his life and find the person who is Whitesnake's user.

Tagging along with Weather Report,[20] she goes on a mission to retrieve Star Platinum's disc and gives it to the Speedwagon Foundation's messenger pigeon for his recovery, succeeding after tricking Whitesnake.

[21] Jolyne gets herself send to the Ultra Security House Unit to find Dio's bone,[22] forced to fight against a couple of Stand users.

[23][24][25] She and Narciso found the Green Baby, formed after Dio's plan in the diary has enacted and chased it to make sure it would not meet Enrico Pucci.

[29][30] With Foo Fighters and Weather Report dead during the journey[28][31] and Jotaro Kujo joining the team, they unsuccessfully fight Pucci in Cape Carneval, who finally evolves his Stand to Made in Heaven.

[35] Pucci halts time's acceleration prior to creating a complete cycle in order to kill Emporio, but ultimately dies at the child's hands instead, despite activating Made in Heaven once more.

Caitlin Moore of Anime News Network enjoys the appearance of a female JoJo and praises Jolyne's multiple states of emotions without coming across as contradictory and how she and Erina Pendleton show similar development as they are forced to toughen up in their given situations.

[3] Alastair Johns of Comic Book Resources sees Jolyne's impression of being a hot-blooded teenager to be a subversion thanks to becoming a cautious and exploitative person.

[50] Cold Cobra of Anime UK News notes how Jolyne is introduced as a meek person before gradually becoming more confident and used to her harsh situation, making her a fun character to cheer for.

[51] Luke Maguire of FictionTalk sees her as a strong female lead not because of her strength, but because of how her character is developed and finds how she uses her Stand ability creatively as brilliant.

Toussaint Egan of Polygon notes how Jotaro and Jolyne are not that different from each other in their respective parts and how his cold personality and parental abandonment made her resent him.