Josefov (also Jewish Quarter; German: Josefstadt) is a town quarter and the smallest cadastral area of Prague, Czech Republic, formerly the Jewish ghetto of the town.

In 1262, Přemysl Otakar II issued a Statuta Judaeorum which granted the community a degree of self-administration.

The ghetto was most prosperous towards the end of the 16th century when the Jewish Mayor, Mordecai Maisel, became the Minister of Finance and a very wealthy man.

Most of the quarter was demolished between 1893 and 1913 as part of an initiative to model the city on Paris.

What was left were only six synagogues, the old cemetery, and the Old Jewish Town Hall (now all part of the Jewish Museum in Prague and described below).Currently Josefov is overbuilt with buildings from the beginning of the 20th century, so it is difficult to appreciate exactly what the old quarter was like when it was reputed to have over 18,000 inhabitants.