The Russian Orthodox Church considers him a saint (along with his most notable opponent, Nilus of Sora); his memory is celebrated on 9 September and 18 October (dates in the Julian Calendar).

Later in his life he severed his relations with the opposition and took the side of the grand prince, sealing this alliance by transferring Joseph-Volokolamsk Monastery to the patronage of Vasili III in 1507.

Joseph Volotsky addressed a number of epistles to the nestiazhateli, in which he tried to prove the legality of monastic landownership and to justify the rich décor of churches.

In his major work, called The Enlightener (Просветитель), which consisted of 16 chapters, he tried to prove the wrongfulness of the "new teaching" in order to be able to prosecute the heretics and convince people not to believe in the sincerity of their repentance.

Taking inspiration from the Roman-Byzantine treatment of heresiarchs and the Dominican-led persecutions in Spain and Portugal, he called for a civil inquisition against heretics and championed their imprisonment and execution.