This was the first and longest-lasting Guale rebellion in La Florida, and ended with the execution of Juanillo by a group of Native American allies of the Spanish, led by Chief Asao.
[2][3][4] After the arrival of the Spanish colonizers in Florida, some chiefs of the Guale tribe, whose vast territory stretched from the Altamaha River to what is now Port Royal, were concerned about the spread of Christianity.
Their grievances under the administration of the Spanish governor, Gonzalo Méndez de Canço, included the Franciscan missionaries forbidding the Indian practices of polygamy, divorce, dancing, games, and tribal wars.
The next day Juanillo called on the chiefs of the other Native American groups who were being expelled from their lands by the Spanish, and incited them to kill missionaries living in the region.
[9] De Canço's troops suppressed the rebellion, which ended decisively when an expedition of Indian allies of the Spanish, led by the mico (chief) of Asao, attacked Juanillo's stronghold in the stockaded town of Yfusinique, killing him with 24 of his main supporters.